Figure 6

BJcuL effects on human neutrophil spontaneous apoptosis. Neutrophils were incubated for 12, 18 and 24 h at 37°C in the presence of BJcuL (2.5 ng/ml), labeled with Annexin-V/PI and analyzed by flow cytometry (A). Results are shown as means ± SD of the percentage of Annexin-V+/PI+ cells from three independent experiments (B). p < 0.05, Student's t-test.