Concentrations of IFNγ and IL-4 in the supernatants of splenocyte cultures from babassu aqueous extract (BAE)-immunized BALB/c mice. Splenocytes (prepared from spleens collected ten days after the end of the immunization) were stimulated with BAE (A and B), or unstimulated (C and D), and the concentrations of IFNγ (A and C) and IL-4 (B and D) in the supernatants were measured by ELISA. Groups of five or six animals were immunized by two subcutaneous injections of 250 μg of BAE, 14 days apart (BAE). Negative control groups of five or six animals were injected with a saline solution in place of the immunizing injections (Saline). The boxes represent the 10-90 percentile intervals and the median values of the results, and the vertical bars the value ranges. P < 0.001, Kruskal-Wallis test. *, P ≤ 0.004; ns, non significant; Mann-Whitney U test.