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Figure 5 | BMC Immunology

Figure 5

From: Differential utilization of NF-kappaB RELA and RELB in response to extracellular versus intracellular polyIC stimulation in HT1080 cells

Figure 5

Involvement of IKK-2 in gene activation in response to extracellular and intracellular polyIC treatments. HT1080 cell line was treated with 40 μg/ml of ex-polyIC and 0.4 μg/ml of in-polyIC for 4 and 8 hours in the absence/presence of IKK-2 inhibitor. Gene expression was measured using qPCR. Graphs show the average of two or three independent experiments, and the student's t-test was performed to indicate statistically significant differences between untreated control and IKK-2 inhibitor-treated cells. (* is for P-value≤0.05, ** is for P-value≤0.01 and NS is 'not significant'.) A. IFNB and IL8 inductions in response to in-polyIC treatment are not significantly affected by IKK-2 inhibition whereas the same gene inductions in response to ex-polyIC treatment are affected by IKK-2 inhibition. B. TNF and CCL3 inductions in response to both ex-polyIC and in-polyIC are relatively not affected by IKK-2 inhibition. C. IL6 and CCL2 inductions in response to both ex-polyIC and in-polyIC are affected by IKK-2 inhibition.

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