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Figure 1 | BMC Immunology

Figure 1

From: Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells

Figure 1

Construction of pEGFP-3CLpro and pEGFP-C145A expression vectors. (A) Scheme of pEGFP-3CLpro and pEGFP-C145A construction. PCMV, human cytomegalovirus (CMV) immediate early promoter; EGFP, enhanced green fluorescent protein gene; MCS, multiple cloning sites; 3CLpro, SARS-CoV 3CLpro gene; C145A, 3CLpro C145A mutant gene; myc, myc-tag sequence; pA, SV40 early mRNA polyadenylation signal sequence. (B)The constructs were digested with restriction enzyme and separated on 1% agarose gel. M, DNA markers.

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BMC Immunology

ISSN: 1471-2172

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