Figure 2
LPS-induced TLR4 expression in β-cells. 5 × 105 human β-cells from adherent cultures were treated with LPS (5 and 50 ng/mL) and analyzed after 48 h. Expression of (a) TLR4 and (b) CD14 mRNA is shown as mean ± SD (3 independent experiments) of relative fold increase in gene expression at 48 h, compared to control (untreated) cells. Statistically significant differences are shown as (*) p < 0.05 and (**) p < 0.005.