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Table 1 Outcomes of islet isolation from pancreata used in this study

From: Toll-like receptor 4 (TLR4) expression in human and murine pancreatic beta-cells affects cell viability and insulin homeostasis

Pre-isolation Post-isolation
Isolation procedure Age Sex Glycemic levels Total IEQ(1) Purity(2) Viability(3) TLR4 expression
    (mg/dL)     mRNA(4) Correlation(5)
PHum1 42 M 186 143,000 70% 80% Yes No
PHum2 52 F ND 116,000 80% 80% Yes No
PHum3 42 M 109 50,000 50% 70% Yes Yes
PHum4 41 M 149 73,000 70% 60% Yes No
PHum5 56 F 140 66,160 60% 50% Yes Yes
PHum6 55 M 143 204,458 90% 90% Yes No
PHum7 54 M 164 23,000 40% 40% Yes Yes
PHum8 53 F 113 60,000 70% 70% Yes Yes
PHum9 55 F 193 93,333 80% 80% Yes No
PHum10 35 M 162 555,125 90% 90% No No
PHum11 55 F 293 380,233 80% 90% Yes No
PHum12 49 F 93 48,000 60% 50% Yes Yes
  1. (1) Each IEQ (islet equivalent) represents one islet 150 μm in diameter, (2) Purity is defined by percentage of Dithizone-positive cells (beta cells) in the islet preparations, (3) Viability as measured by live/dead fluorescence, (4) Relative TLR4 mRNA expression levels are variable and this column indicates only presence (Yes) or absence (No) of detectable levels of TLR4 mRNA at any time point, (5) Correlation was obtained from time-course analysis of mRNA expression of TLR4 and β-cell death-associated molecules by the Pearson coefficient test. The presence (Yes) or absence (No) corresponding to a significant positive or negative linear correlation (-0.9 <r > 0.9, p > 0.05) is indicated in this column.