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Table 1 Outcomes of islet isolation from pancreata used in this study

From: Toll-like receptor 4 (TLR4) expression in human and murine pancreatic beta-cells affects cell viability and insulin homeostasis

Pre-isolation

Post-isolation

Isolation procedure

Age

Sex

Glycemic levels

Total IEQ(1)

Purity(2)

Viability(3)

TLR4 expression

   

(mg/dL)

   

mRNA(4)

Correlation(5)

PHum1

42

M

186

143,000

70%

80%

Yes

No

PHum2

52

F

ND

116,000

80%

80%

Yes

No

PHum3

42

M

109

50,000

50%

70%

Yes

Yes

PHum4

41

M

149

73,000

70%

60%

Yes

No

PHum5

56

F

140

66,160

60%

50%

Yes

Yes

PHum6

55

M

143

204,458

90%

90%

Yes

No

PHum7

54

M

164

23,000

40%

40%

Yes

Yes

PHum8

53

F

113

60,000

70%

70%

Yes

Yes

PHum9

55

F

193

93,333

80%

80%

Yes

No

PHum10

35

M

162

555,125

90%

90%

No

No

PHum11

55

F

293

380,233

80%

90%

Yes

No

PHum12

49

F

93

48,000

60%

50%

Yes

Yes

  1. (1) Each IEQ (islet equivalent) represents one islet 150 μm in diameter, (2) Purity is defined by percentage of Dithizone-positive cells (beta cells) in the islet preparations, (3) Viability as measured by live/dead fluorescence, (4) Relative TLR4 mRNA expression levels are variable and this column indicates only presence (Yes) or absence (No) of detectable levels of TLR4 mRNA at any time point, (5) Correlation was obtained from time-course analysis of mRNA expression of TLR4 and β-cell death-associated molecules by the Pearson coefficient test. The presence (Yes) or absence (No) corresponding to a significant positive or negative linear correlation (-0.9 <r > 0.9, p > 0.05) is indicated in this column.