Figure 2From: Detection of autoantibodies against reactive oxygen species modified glutamic acid decarboxylase-65 in type 1 diabetes associated complicationsDetermination of apparent association constants for ROS-GAD 65 by Langmuir plot. Langmuir plot of reciprocal of bound antigen concentration to antibody (1/r) versus reciprocal of free antigen concentration (1/[Ag]). Antigen and antibody binding between ROS-GAD65 and IgG isolated from nephropathic (-■-) serum no.3, retinopathic (-●-) serum no. 6 of complicated T1D and uncomplicated (-▲-) (serum no. 11) T1D subjects. Each value represents mean ± SD of four independent assays.Back to article page