Detection of autoantibodies against reactive oxygen species modified glutamic acid decarboxylase-65 in type 1 diabetes associated complications

Table 1 Inhibition of serum IgG from uncomplicated T1D, complicated T1D and control subjects towards native and modified GAD₆₅.

Maximum percent inhibition at 20 μg/ml
Serum	Uncomplicated T1D		Nephropathic T1D		Retinopathic T1D		Controls
	N-GAD₆₅	M-GAD₆₅	N-GAD₆₅	M-GAD₆₅	N-GAD₆₅	M-GAD₆₅	N-GAD₆₅	M-GAD₆₅
1	36	54	40	78	39	85	-	-
2	38	40	42	66	37	79	-	7
3	47	61	54	79	44	57	7	11
4	37	55	41	70	41	68	-	-
5	43	49	48	66	32	65	-	-
6	35	54	35	74	37	79	-	10
7	40	60	44	56	43	76	10	7
8	26	68	39	73	51	57	-	-
9	22	37	30	59	36	70	7	9
10	38	56	37	62	50	77	8	5
11	45	63	42	57	40	70	-	7
12	34	50	29	59	48	69	-	-
13	46	55	37	76	50	79	5	9
14	41	52	36	69	36	75	-	-
15	33	39	39	78	33	81	-	-
16	21	48	28	79	30	83	6	7
17	19	41	26	69	28	78	8	6
18	26	37	27	75	37	79	6	7
19	31	45	35	81	35	82	9	8
20	28	47	39	80	40	80	-	-
Mean ± SD	35.2 ± 5.9	50.6 ± 7.2	39.2 ± 5.4	70.3 ± 8.2*	41.1 ± 5.3	74.5 ± 6.5*	7.3 ± 3.6	7.2 ± 3.7

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