Figure 3

OK432 stimulated DC secrete higher quantities of cytokines and chemokines compared to cytokine cocktail treated DC. The DC were stimulated for 24 hours and the amount of cytokines/chemokines secreted were analysed using a 25-plex bead assay and a sandwich ELISA for IL-12p70, IL-23 and TGF-β1. Only cytokines and chemokines with statistically (group wise analyses with Mann-Whitney) different concentrations between DC stimulated with cytokine cocktail and 0.1 KE OK432 are shown, with the exception of IL-8. No significant difference was found for IL-1β, IL-2, IL-5, IL-13, TGF-β1 and TNF-α. GM-CSF and IL-4 were part of the added cytokines and therefore not considered in the analysis. The concentrations are given in pg/ml. See figure 1 for figure key.