Figure 5
DC matured with OK432 are exceeding the cytokine cocktail treated DC in allogeneic T-cell stimulation. DC were matured for 24 hours with the indicated stimuli, washed, irradiated, and 2×104 DC were co-cultured with 2×105CFSE labelled allogeneic leukocytes that had the monocyte population removed, for 5 days. The cells were harvested, stained for CD4 and CD8, and analysed on a flowcytometer. Panel A show the negligible effect the 0.1 KE OK432 compound has on the T-cell proliferation in an allogeneic leukocytes culture in the absence of direct DC stimulation. Panel B shows the effect immature, cytokine cocktail and OK432 stimulated DC have on the total NK cell population (CD3-CD16+CD56+/-) within an allogeneic leukocytes culture that had the monocyte population removed. Similar results were found using forty thousand DC in the co-culture (data not shown). Histograms from one experiment are shown in C and D. Panel E and F show the median percentage compared to cyto (from the same donor) set at 100 percent plus interquartile range in division number from 0 to 7, for CD4 and CD8 positive cells. (n = 4). DC treated with OK432 induced more proliferation of both CD4 and CD8 positive cells and had a high proportion of cells with the maximum detectable divisions. Figure key: Imm: Immature DC; Cyto: cytokine cocktail treated DC; OK432: 0.1KE OK432 treated DC.