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Figure 2 | BMC Immunology

Figure 2

From: Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature

Figure 2

Gene expression analysis of CD8+ SP T cells obtained from HSCs cultured on OP9-DL1 cells. Gene expression analysis by QRT-PCR from coculture-derived CD8 SP T cells (CD8+ CD4- CD3+). CD8 SP, CD4 SP T cells and CD33+ myeloid cells were purified from the Lin+ fraction of UCB samples and served as controls. Transcript levels for the indicated genes were normalized to human β-actin, and these data are representative of 3 independent experiments, with the standard error bars shown corresponding to values obtained from triplicate wells within an individual experiment.

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