Figure 1

TLR2 expression profile on fresh and cultured immune cells. (A) Dendritic cells (CD11c⁺), CD4⁺CD25⁺Foxp3⁺ Treg and CD4⁺ effector T cells (Foxp3^-) were isolated from spleens of naïve mice and immediately analyzed for TLR2 surface expression by flow cytometry (grey line, isotype control; black line, TLR2 antibody). TLR2 is expressed on freshly isolated DCs, while expression on Teff and Treg is absent or low. (B) Treg and Teff were cultured in the presence of Pam3Cys, anti-CD3 and irradiated splenocytes as APCs for several weeks (see materials and methods). Activation induced TLR2 expression on both Treg and Teff which remained high when cultured for several weeks. Representative results of 3 independent experiments are shown, all experiments were performed in duplicate.