Figure 3

Cytokine production of Teffs upon TLR2-triggering. Freshly isolated and cultured Teff were stimulated with anti-CD3 and increasing concentrations of Pam3Cys. Fresh Teff were additionally cultured in the presence of irradiated APCs that did not produce these cytokines upon stimulation (data not shown). Freshly isolated Teff showed a clear dose dependent increase in IL-2, IL-4, IL-10 and IFNγ. Cultured Teff cells produced high amounts of cytokines in the absence of Pam3Cys and failed to show a clear effect of Pam3Cys. All cytokine profiles were determined by Luminex analyses. Representative results of 2 independent experiments are shown, all experiments were performed in duplicate.