Figure 2

Activating markers are expressed differently in spleen and liver DX5⁺NKT cells. Detection of NK cell markers confirmed differences between spleen and liver subsets of DX5⁺NKT cells in NK1.1^-Balb/c mice. CD94 expression was higher in liver DX5⁺NKT cells (A). Furthermore, the liver subset revealed less expression of maturation marker CD103 and CD62L compared to spleen (B). The activating markers CD154 and CD178 were in both freshly-isolated cell subsets only little expressed (C). Upon stimulation spleen DX5⁺NKT cells displayed an up-regulation of CD154, whereas the liver subset did not. Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).