Figure 2

OVA _323-339 mutations reduce antigenicity for T cells. A, Summary of OVA_323-339 mutations, their predicted MHC II binding affinities, and experimental result from tetramer competition assays. Percent of tetramer binding was calculated as: number of tetramer-positive T cells in the presence of a competing peptide epitope / number of tetramer-positive T cells in the absence of a competing peptide epitope ×100%. B, Proliferation of CFSE-labeled DO11.10 CD4⁺ T cells co-cultured for 4 days with tolerogenic DCs presenting an indicated epitope. The line plots summarize the results from three independent experiments. **, p < 0.01, n = 3.