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Figure 4 | BMC Immunology

Figure 4

From: Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells

Figure 4

Adoptive transfer of CD25- iTreg cells suppresses T cell response in recipient mice. CD4+CD25- T cells from OVA323-339, MT1, or MT2 co-immunized, or from naïve Balb/c, were adoptively transferred to naïve Balb/c. The activity of the donor CD25- iTreg was assessed by sensitizing the recipients with OVA323-339 in IFA. A, CD4+ T cells were isolated from the recipient after sensitization. The cells were labeled with CFSE and restimulated with OVA323-339 in culture. Divided cells were identified by CFSE dilution and counted by flow cytometry. The result is expressed as a percent of total CFSE+ T cells. Shown is one of three independent experiments of similar results. B, CD4+ T cells were isolated from the recipients after sensitization and intracellularly immunostained for IFN-γ. IFN-γ+CD4+ T cells were counted by flow cytometry and calculated as a percent of total CD4+ T cells. Shown is one of three independent experiments of similar results. C &D, IFN-γ and IL-10 secretion in the supernatant of restimulated T cells. Anti-CD3 mAb (KT3) or KT3 + IL-2 + IL-4 was used in positive controls for induction of indicated cytokines. Shown is one of three independent experiments of similar results. *, p < 0.05, **, p < 0.01, n ≥ 5. Difference was calculated among all groups (A, B) or among Co323, CoMT1, and CoMT2 (C, D).

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