Figure 6

Attenuation of skin reaction by co-immunization-induced CD25^- iTreg. A, Flea antigen stimulated T cell proliferation. B, In vivo T cell response induced by flea-specific i.d. test. C, H&E staining of skin section. The black arrows indicate infiltrating T cells. D, Mast cell number and degranulation (black arrow) by Toluidine Blue staining. E, Seven days after co-immunizaiton, CD25^- iTreg cells were counted as a percentage of CD4⁺CD25^- T cells. Shown is one of three independent experiments with similar results. *, p < 0.05; **, p < 0.01, n ≥ 5. Difference was calculated among all immunized groups (A, B) or all groups (E).