Figure 2
From: Production of antibodies with peptide-CpG-DNA-liposome complex without carriers
Effect of CpG-DNA encapsulated in DOPE:CHEMS on intracellular uptake and endosomal localization of CpG-DNA. (a) Effect of DOPE:CHEMS (1:1 ratio) encapsulation on uptake of CpG-DNA was analyzed by FACS. RPMI 8226 cells and RAW 264.7 cells were treated with FITC-labeled CpG-DNA or FITC-labeled CpG-DNA encapsulated in DOPE:CHEMS complex for 10 min. Intracellular uptake of CpG-DNA was measured by FACS analysis. 4531(O), FITC-labeled MB-ODN 4531(O); Lipoplex(O), FITC-labeled MB-ODN 4531(O) encapsulated in DOPE:CHEMS complex. (b) Involvement of endosomal acidification in IL-8 promoter activation induced by CpG-DNA encapsulated in DOPE:CHEMS complex was confirmed by chloroquine pretreatment. Luciferase activity in was measured in relative light units, and the value was normalized to Renilla activity. The results are represented in terms of fold activation and are compared to the PBS-treated control. Each bar represents Mean ± SD values obtained from three experiments. **P < 0.01 (vs PBS control).