Figure 4

Production of Th1-associated HEL (or OVA) antigen-specific IgG induced by CpG-DNA encapsulated in DOPE:CHEMS complex. (a) Amounts of anti-HEL-specific total IgG and IgG isotypes. (b) Titers of anti-HEL-specific total IgG, anti-HEL-specific IgG1, and anti-HEL-specific IgG2a. (c) Amounts of anti-OVA-specific total IgG and IgG isotypes. (d) Titers of anti-OVA-specific total IgG, anti-OVA-specific IgG1, and anti-OVA-specific IgG2a. Three BALB/c mice were immunized with one of the following combinations: 50 μg of HEL (or OVA); 50 μg of MB-ODN 4531(O) and 50 μg of HEL (or OVA); 50 μg of HEL (or OVA) with DOPE:CHEMS; 50 μg of Lipoplex(O) and 50 μg of HEL (or OVA); or 50 μg of Lipoplex GC(O) and 50 μg of HEL; IFA and 50 μg of HEL (or OVA). The sera were collected, and the total IgG and IgG isotypes were assayed with an ELISA kit. Each bar represents Mean ± SD values obtained from three mice. This experiment was performed 3 times with similar results. **P < 0.01 (vs IFA + HEL (or OVA)).