Figure 7
From: Production of antibodies with peptide-CpG-DNA-liposome complex without carriers
Detection of TM4SF5 by anti-hTM4SF5 monoclonal antibody produced by hTM4SF5R2-3 peptide and Lipoplex(O) complex. (a) Reactivity of anti-hTM4SF5R2-3 monoclonal antibody with hTM4SF5R2-3 peptide. hTM4SF5R2-3 peptide was immobilized on the plate, and competitive ELISA was performed using increasing amounts of soluble hTM4SF5R2-3 peptide. (b) Expression of hTM4SF5 mRNA in the human hepatocarcinoma cell lines. The expression level of hTM4SF5 mRNA was analyzed by RT-PCR with cDNAs from the indicated cell lines and normal hepatocytes. (c-d) Detection of hTM4SF5 by anti-hTM4SF5R2-3 peptide monoclonal antibody in human hepatocarcinoma cell lines. (c) The expression of hTM4SF5 protein was analyzed by immunoblotting with anti-hTM4SF5R2-3 peptide mAb in human hepatocarcinoma cell lines. (d) The hepatocarcinoma cell lines were stained with anti-hTM4SF5R2-3 peptide monoclonal antibody and then FITC-conjugated antibody to anti-mouse IgG, and analyzed with a FACScan flow cytometer. Normal mouse IgG was used as a control.