Figure 8
From: Production of antibodies with peptide-CpG-DNA-liposome complex without carriers
Functional effects of anti-hTM4SF5 monoclonal antibody produced by immunization with hTM4SF5R2-3 peptide and Lipoplex(O) complex. (a) Effect of anti-hTM4SF5R2-3 peptide monoclonal antibody on cell growth. Cell growth was measured by MTT assay. Each bar is expressed as a Mean + SD of three experiments. **P < 0.01 (vs normal IgG control). (b) Effect of anti-hTM4SF5R2-3 peptide monoclonal antibody on cell cycle. The cells were stained with propidium iodide in PBS containing RNase and analyzed by flow cytometry. (c) Effect of anti-hTM4SF5R2-3 peptide monoclonal antibody on proliferation. The DNA synthesis activity was monitored by BrdU incorporation assay. Each bar is expressed as a Mean ± SD of three experiments. *P < 0.05, **P < 0.01 (vs normal IgG control). (d) Effect of anti-hTM4SF5R2-3 peptide monoclonal antibody on apoptosis. FACSCalibur flow cytometer was used to analyze cells containing fluorescein-labeled double strand DNA breaks. As a positive control, the cells were treated with DNase I prior to analysis. These experiments were performed three times with similar results.