Figure 2From: Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cellsThymocyte stimulation response. Thymocytes were stimulated with CD3-antibody for 2 and 5 minutes and analyzed by either immunoprecipitation with phosphotyrosine antibody followed by Western blot or by just performing Western blot on cell lysates. Blots were probed for phosphotyrosine, phospho-PLCγ, phospho-Vav, phospho-Zap70, phospho-Cbl, phospho-ERK, Cbl, ERK and Shb.Back to article page