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Figure 4 | BMC Immunology

Figure 4

From: Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cells

Figure 4

Peripheral T cell signaling and proliferation. (A) The number of splenocytes was estimated by counting cells in a Bürker chamber (n = 10, mean ± SEM). (B) Splenocytes were separated into CD4+ and CD8+ cells and stimulated for 96 hours with CD3- and CD28 antibodies. Radioactively labeled thymidine was added for the last 4 hours to estimate proliferative activity. The proliferation data is presented as a percentage of the highest value in each experiment that for CD4+ cells corresponds to 264326 ± 41432 and for CD8+ cells to 191290 ± 49852 DPM (n = 10, mean ± SEM, * denotes p < 0,05 by paired Students t-test). (C) CD4+ splenocytes were stimulated for 2 minutes with CD3 antibody and subsequently analyzed by Western blot. The samples were probed with phosphotyrosine,- phospho- p38-, phospho-ERK- and ERK antibodies.

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