Figure 6
From: Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cells
Naïve and memory CD4+ T cell profile. (A) Isolated splenocytes were stained for CD4, CD62L and CD44 in order to identify naïve and memory T cells (n = 6, mean ± SEM). (B, C) Naïve CD4+CD62L+ T cells were isolated and stimulated for 72, 96 or 120 hours followed by 2 hours of BrdU-pulse labeling. Cell cycle status was subsequently assessed based on BrdU and 7AAD staining. Plots represent typical experiment (n = 6, mean ± SEM, * denotes p < 0.05 by paired Students t-test). (D) The numbers of stimulated naïve CD4+CD62L+ T cells were determined at the indicated time points and related to the initial numbers of cells plated to determine relative increase in cell numbers (n = 6, mean ± SEM, * denotes p < 0.05, ** denotes p < 0.01 by paired Students t-test).