Figure 7
From: Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cells
T H cell gene expression profile in unfractionated naïve CD4+CD62L+ T lymphocytes in response to TCR stimulation. (A, B, C) Transcript levels of cytokines IL-2, IL-4 and IFN-γ were determined by semiquantitative RT-PCR and normalized against the expression of the house keeping gene β-actin. Data is presented as 20 minus the mean of the normalized CT values ± SEM. (n = 6, ** denotes p < 0,01 by paired Students t-test). (B, C, D) Relative expression of the transcription factors GATA3 and T-bet were estimated through a semiquantitative RT-PCR and normalized against the expression of the house keeping gene β-actin. GATA3 is presented as 30 minus the mean of the normalized CT values and Tbet as 20 minus the mean of the normalized CT values ± SEM (n = 6, * denotes p < 0,05 by paired Students t-test).