Figure 3From: Expression of neuroimmune semaphorins 4A and 4D and their receptors in the lung is enhanced by allergen and vascular endothelial growth factorRegulation of Sema4A and Sema4D expression on lung immune cells by allergen and VEGF. Flow cytometric detection of Sema4A and Sema4D expression on lung and spleen DC (A-B), lung T and B cells (C), and MHCII+ cells (D). Single cell suspensions were prepared as described in Methods. Conventional DC were identified by staining cells with anti-CD11c, -MHCII, and -CD11b Abs used for lung cDC detection. Highly fluorescent macrophages were gated out from the further analysis as large cells on FSC-SSC. (A) No cell surface Sema4A and low Sema4D expression was found on lung cDC. Spleen CD11c+MHCIIneg DC subset demonstrated high Sema4A and Sema4D expression which was further upregulated by allergen. (B) Intracellular Sema4A was targeted to a specific population of CD11cintermed/MHCIIlow cells under inflammatory conditions. (C-D) Low Sema4A and high Sema4D expression was detected on lung B220+ cells, CD3+ cells, and MHCII+ cells.Back to article page