PS-G increased co-stimulatory molecule expression on MD-DCs. Immature MD-DCs were pulsed either with Der p 1 (1 μg/ml) or PS-G (10 or 25 μg/ml), or both for 24 h and analyzed by flow cytometry for CD80, CD86, CD83, and HLA-DR expression. DCs stimulated with LPS (100 ng/ml) were positive maturation controls. Values shown are the mean fluorescence intensity (MFI) indexes. Dotted line, isotype control; solid line, specific mAbs. One representative of three independent experiments is shown.