Figure 5

LPS-induced STAT3 phosphorylation was inhibited by IL-10 neutralizing mAb on DCs. DCs from Lewis rats were stimulated with LPS (0.5 μg/ml) in the presence of control IgG (Ctrl IgG, 2 μg/ml), anti-IL-10 mAb (2 μg/ml) or anti-IL-10 mAb combined with Shc silencing for 6 h. Cells were harvested and Western blot analysis was performed for phospho-STAT3 at Tyr-705 and for total STAT3. Anti-IL-10 mAb significantly inhibited the LPS-induced STAT3 phosphorylation in both DCs and Shc-silenced DCs indicated the dependence of secreted IL-10 in STAT3 activation. Each column represents the mean ± SE of the three independent experiments (n = 3; *, P < 0.05).