Figure 11

Tumor-derived GM-CSF and mDC cultures. (A) Expression of GM-CSF by mouse ovarian tumor cells at the level of RNA as determined by qualitative PCR. An experiment representative of 2 independent experiments is shown. (B) Expression of GM-CSF by several mouse ovarian tumors at the level of RNA as determined by quantitative real time PCR. Samples were analyzed in quadruplicate. All samples except tumor 7 (* p < 0.05) expressed GM-CSF at levels that were not significantly different from those detected in spleen, an immune organ that was used as a control for these studies. (C) Staining of mouse ovarian tumors shows heavy DC infiltration. Immunohistochemistry analysis (200 × magnification). A photograph representative of 8 stained tumors is depicted. (D) Myeloid DCs were cultured on polystyrene and fibronectin in the presence of RPMI supplemented or not with GM-CSF, and RPMI supplemented with 30% tumor conditioned medium without addition of GM-CSF in the presence of anti-GM-CSF receptor antibody (4 μg/ml) or isotype control. (20 × magnification). An experiment representative of 2 independent experiments with n = 2 is shown.