Figure 12
Expression of surface markers on mDCs cultured for 1 week in the presence of tumor factors. Myeloid DCs were cultured on polystyrene and fibronectin in the presence of RPMI medium supplemented with 30% tumor conditioned medium without addition of external GM-CSF and in the presence of anti-GM-CSF receptor antibody (4 μg/ml) or isotype control. Cells were recovered from cultures after 1 week and analyzed by qualitative flow cytometry. Grey histograms represent isotype controls. Quadrants in dot plot graphs were defined using isotype controls. An experiment representative of 2 independent experiments (n = 2 each experiment) is shown.