Figure 7
One-week mDC cultures express angiogenic molecules. (A) VEGF was detected by ELISA analysis on mDC-culture supernatants. The ELISA assay specifically recognized murine VEGF. Data from 5 independent experiments (n = 2 for each condition in every experiment) were pooled for this analysis. No significant differences were obtained in the EGM or RPMI groups as determined by ANOVA analysis. However the EGM group as a whole showed significantly lower VEGF levels than the RPMI group as determined by ANOVA analysis. (B) FGF ELISA analysis of RPMI cultures. Variable expression of this molecule was observed in repeated experiments. Being the ELISA assay able to recognize human FGF, a component of the EGM media, only RPMI supernatants were evaluated. Data from 5 independent experiments (n = 2 for each condition in every experiment) were pooled for this analysis. Only gelatin cultures showed significantly higher levels of FGF when compared to the polystyrene control as determined by ANOVA analysis followed by Dunnett's post comparison test. (C) CD54 expression in 1-week cultures. A qualitative flow cytometry analysis was performed in order to determine the expression of CD54 in the surface of these cells. The experiment was repeated 3 times with similar results. Grey histograms represent isotype controls.