Figure 9

GM-CSF and mDC expression of surface molecules by cells cultured with RPMI. Myeloid DCs were cultured for 1 week on different surfaces in RPMI in the presence or absence of 3 ng/ml of GM-CSF. Attached cells were recovered and the expression of CD11c, CD11b, GR1, MHC-II and CD80 analyzed by qualitative flow cytometry. An experiment representative of 2 to 5 independent experiments is shown. Grey histograms represent isotype controls. Quadrants in dot plot graphs were defined using isotype controls.