Figure 2

Gating strategy used for the 10-color flow cytometric stain. Thymocytes were phenotyped by flow cytometry with Annexin-V-FITC and mAbs against mouse CD4, CD8, CD25, CD28, CD44, CD117, CD24, TCR-β and either IL-4Rα (CD124) or IL-6Rα (CD126). Only singlets and Annexin-V negative cells were included in the analysis. Cells of other lineages were excluded using mAbs against the markers NK1.1, Ly6, Ter119, CD19, CD11b, and TCR-γδ. Arrows show the logical gating progression used to achieve discrimination of the 21 subsets. Forty million thymocytes were stained to generate this figure.