Purification of the AFP domains. (A) SDS-PAGE of total lysates without or with beta-gal induction (control and induction were denoted as - or + respectively). Gels were stained with Coomassie blue. M denotes protein marker. (B) Coomassie stained gels of purified proteins from each domain with molecular weight of 24.8 kDa (D1) and 24.4 kDa for D2 and D3. (C) Gel from B) was transferred to the nitrocellulose membrane and blotted with an anti-His antibody.