FL-AFP reduced the viability of MDDC population at high concentrations. Addition of AFP 12.50 μg/ml or above resulted in a significant cell death of MDDC population based on propidium iodide (PI) assay. PI staining was performed on MDDC culture at day 8, and analyzed with flow cytometry. The viability of MDDC was determined as percentage of negative PI cells. The graphics shown are a representative of three experiments with similar results.