Figure 3
From: Detection of autoreactive CD4 T cells using major histocompatibility complex class II dextramers
IAs/PLP 139-151 dextramers, but not tetramers, labeled with FITC are useful to detect antigen-specific cells. PLP-reactive cells generated from SJL mice immunized with PLP 139-151 were stained with PLP 139-151 or control tetramers and dextramers, each labeled with FITC, PE or APC, followed by anti-CD4 and 7-AAD. After acquiring the cells by FC, percentages of tet+ or dext+ cells were analyzed in the live (7-AAD-) CD4 subset. Data from one of the two independent experiments each involving 2 mice are shown.