Figure 7

Fibronectin regulates Treg differentiation. T lymphocytes (CD4+CD25-) were obtained from the mesenteric lymph nodes of mice and exposed to plate bound anti-CD3 (10 mg/ml) and fibronectin, as well as soluble anti-CD28 (1 mg/ml), rIL-2 (100 u/ml) and TGFβ (10 ng/ml) as described in materials and methods. After 24 h the proportion of FoxP3+ cells were determined using FACS analysis. In A, i: representative neutral, 1000 ng/ml fibronectin; ii: iTreg, 0 ng/ml fibronectin; iii: iTreg, 125 ng/ml fibronectin; iv: iTreg, 1000 ng/ml fibronectin. The barchart in B represents the overall dose-response relationship, performed in triplicate and repeated twice. (*p < 0.05, **p < 0.01, using ANOVA).