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Table 2 Cytokines levels in lymphnode cell cultures from infected BALB/c mice after coincubation with synthetic peptides

From: In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection

pg/mL

μM

 

IL-4

IL-10

IL-12

IFN-γ

NO

Con A

979.69 ± 3.79

590.30 ± 77.20

32.16 ± 5,21

872.33 ± 17.12

7.78 ± 3.49

PLA

37.82 ± 5.67

188.30 ± 34.88

12.02 ± 11,77

474.92 ± 92.24

0.84 ± 0.13

P1

ND

ND

75.00 ± 34.65

ND

ND

P2

ND

ND

ND

15.88 ± 3.19

ND

P4

ND

10.97 ± 2.47

ND

49.25 ± 20.10

ND

P5

ND

ND

ND

ND

ND

P6

ND

2.97 ± 0.82

ND

ND

ND

P7

ND

ND

ND

ND

ND

P8

ND

ND

ND

ND

ND

  1. Results presented are from a representative assay from three independent repeats using lymph nodes from 15 infected mice. Results are presented as mean ± standard deviation values. ND - below the threshold established by control group.