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Table 3 Cytokines levels in lymphnode cell cultures from infected CBA mice after coincubation with synthetic peptides

From: In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection

pg/mL

μM

 

IL-4

IL-10

IL-12

IFN-γ

NO

Con A

226.84 ± 9.53

338.10 ± 11.49

26.85 ± 11.94

939.48 ± 0.00

ND

PLA

33.67 ± 4.82

68.57 ± 6,88

ND

87.06 ± 12,23

1.39 ± 0.96

P2

ND

ND

18.37 ± 11.58

ND

ND

P3

ND

ND

16.44 ± 3.25

ND

ND

P8

ND

27.46 ± 0.74

ND

ND

ND

P9

ND

21.03 ± 0.16

8.02 ± 3.85

ND

ND

  1. Results presented are from a representative assay from three independent repeats using lymph nodes from 15 infected mice. Results are presented as mean ± standard deviation values. ND - below the threshold established by control group.