Effects of CP690, 550 on cytokine genes expression in activated CD4+ T cells. CD4+ T cells were stimulated with CD3 monoclonal antibodies in the presence or absence of CP690,550. After 8-hr or 24-hr stimulation, total RNA was extracted and IL-2 (A), IFN-γ (B), IL-4 (C) and IL-17A (D) mRNA expression was measured by real-time PCR. * p < 0.001 vs CD3 Ab-stimulated lymphocytes. The results show a representative result from three independent experiments.