Figure 2

Siva-binding activity is contained within a central portion of the FOXP3 protein (amino acids 106-332). A. Diagram depicting FOXP3 protein domain organization and truncation mutants that were fused to the Myc C-terminus and used in the Co-IP shown in B. (bd, binding domain; Zn F, zinc finger; L Zip, leucine zipper; Fkh, forkhead). B. Co-immunoprecipitation (Co-IP) to map FOXP3's Siva-binding activity. Co-IPs were performed as described in Figure 1. Binding between the FOXP3 Fkh mutant and Siva was tested three times. The other FOXP3 mutants shown here were tested once.