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Figure 3 | BMC Immunology

Figure 3

From: Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells

Figure 3

The Siva C-terminus is sufficient to bind FOXP3. A. Diagram showing the Siva protein domain organization (SAH, spherical amphipathic helix; DDHR, death domain homology region; B box; and Zn F, zinc finger) and Siva truncation mutants, which were fused to the EGFP C-terminus and tested in Co-IPs shown in B and C. B. Co-IP to map Siva's FOXP3-binding activity (hc, antibody heavy chain; lc, antibody light chain). These Siva truncation mutants were tested three times for FOXP3-binding activity. C. Co-IP to test the FOXP3-binding activity of the Siva B box and ΔB box mutants. These mutants were each tested twice for FOXP3-binding activity.

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