Skip to main content
Figure 5 | BMC Immunology

Figure 5

From: Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells

Figure 5

Siva represses IL-2 promoter activity. To evaluate IL-2 promoter activity, Jurkat T cells were transfected with an IL-2 luciferase reporter plasmid plus other constructs to overexpress or KD Siva expression. IL-2 promoter-driven luciferase activity is expressed as a measure of relative light units (RLUs) normalized to the viable cell number. A. Siva-1 overexpression with pHSPG-Siva-1 repressed IL-2-promoter activity. B. Siva KD by shRNA had an enhancing effect on IL-2 promoter activity. *s indicate statistical difference compared to vector based on p < 0.05 by a two-tailed Student's t-test. These experiments were each performed twice and a result from one representative experiment is shown.

Back to article page