Figure 8

NFκB and NFAT reporter activity in response to Siva-1 and FOXP3 overexpression. Jurkat T cells were transfected with NFκB and NFAT luciferase reporter plasmids and expression plasmids encoding FOXP3 or Siva-1 or the parental vector control, pHSPG (PG) (RLUs, relative light units). A. Both FOXP3 and Siva-1 repress NFκB activity in response to PMA and Ionomycin. B. FOXP3 repressed NFAT activity in response to PMA and Ionomycin. *s indicate statistically significant difference between the indicated bar and the PG vector control based on p < 0.05 by a two-tailed Student's t-test. ** indicates statistically significant difference between Siva-1 and FOXP3 in combination compared to FOXP3 alone. These experiments were repeated twice and data from one representative experiment is shown.