Figure 2
Role of Mac-1 and LFA-1 for leukocyte adhesion in surgically prepared cremaster muscle venules before and after additional fMLP superfusion. Leukocytes adhesion (number of adherent cells/mm2) is shown before and 5 minutes after fMLP-superfusion (10 μM) in surgically prepared cremaster muscle venules of wild type mice (13 venules in 10 mice) and RAGE-/- mice (10 venules in 6 mice) without antibody treatment and after they were systemically treated with blocking antibodies against murine Mac-1 (Tib128; 19 venules in 6 wild type mice and 14 venules in 4 RAGE-/- mice) or LFA-1 (Tib217, 16 venules in 5 wild type mice and 20 venules in 6 RAGE-/- mice), or their respective isotype control antibodies (IgG2b for Mac-1; 11 venules in 4 wild type mice and IgG2a for LFA-1; 12 venules in 4 wild type mice) immediately before starting fMLP superfusion. All values are presented as mean ± SEM and significant differences (p < 0.05) vs. control mice are indicated by the asterisk.