Figure 4
Role of Mac-1 and LFA-1 for leukocyte adhesion in TNF-α-stimulated cremaster muscle venules before and after additional fMLP superfusion. Leukocytes adhesion (number of adherent cells/mm2) is shown before and 5 minutes after fMLP-superfusion (10 μM) in 3 h TNF-α-stimulated cremaster muscle venules of wild type control mice and Icam1-/- mice, which are systemically treated with blocking antibodies against murine Mac-1 (Tib128; 20 venules in 4 wild type mice and 13 venules in 3 Icam1-/- mice), LFA-1 (Tib217, 13 venules in 4 wild type mice and 14 venules in 3 Icam1-/- mice) or their respective isotype control antibodies (IgG2b for Mac-1; 10 venules in 3 wild type mice and IgG2a for LFA-1; 8 venules in 3 wild type mice) immediately before starting fMLP superfusion or without antibody pretreatment (6 venules in 6 wild type mice and 4 venules in 4 Icam1-/- mice). All values are presented as mean ± SEM and significant differences (p < 0.05) vs. WT control mice and Icam1-/- control mice are indicated by the asterisk.