Figure 5

Leukocyte adhesion in CXCL1-stimulated cremaster muscle venules. The number of adherent cells (mean ± SEM) was assessed in exteriorized cremaster muscle venules of RAGE^-/- mice (10 venules in 6 mice), Icam1^-/- mice (11 venules in 5 mice), untreated (12 venules in 7 mice), anti-LFA-1 antibody (10 venules in 4 mice) or isotype antibody (9 venules in 3 mice) treated wild type mice before and five minutes after systemic injection of CXCL1 (keratinocyte-derived chemokine, KC; 600 ng/mouse). Significant differences (p < 0.05) of the number of adherent leukocytes before CXCL1 vs. after injection of CXCL1 are indicated by *, # and § for wild type mice, RAGE^-/- mice and isotype antibody treated wild type mice, respectively.