RAGE and ICAM-1 differentially control leukocyte recruitment during acute inflammation in a stimulus-dependent manner

Table 1 Hemodynamic and microvascular parameters in cremaster muscle venules during trauma induced inflammation and additional fMLP stimulation

	Mice	Venules	Diameter	Centerline Velocity	Wall shear rate	Systemic leukocyte counts
	(N)	(n)	(μm)	(μm/s)	(s-1)	(cells/μl)
Wild type	10	13	33 ± 1	2700 ± 200	2000 ± 100	6600 ± 600
RAGE ^-/-	6	10	33 ± 2	2700 ± 200	2100 ± 100	6800 ± 900
Icam1 ^-/-	4	4	30 ± 2	2400 ± 300	2000 ± 300	6400 ± 300
Wild type+ anti-Mac-1	6	19	30 ± 1	2500 ± 100	2100 ± 100	4800 ± 200
RAGE-^/-+ anti-Mac-1	4	14	29 ± 2	2500 ± 100	2100 ± 150	6700 ± 600
Wild type+ isotype AB	3	9	33 ± 2	2300 ± 100	1800 ± 100	6300 ± 700
Wild type+ anti-LFA-1	5	16	27 ± 1	2400 ± 100	2200 ± 100	6900 ± 700
RAGE-/-+ anti-LFA-1	6	20	30 ± 1	2400 ± 100	2200 ± 100	7900 ± 700
Wild type+ isotype AB	3	9	29 ± 2	2400 ± 100	2200 ± 200	5300 ± 400
			n.s.	n.s.	n.s.	n.s.

Vessel diameter, centerline velocity, wall shear rate and systemic leukocyte counts are presented as mean ± SEM of the investigated venules during trauma-induced inflammation with additional local fMLP superfusion. N.s. = non significant.

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