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Figure 1 | BMC Immunology

Figure 1

From: DC-STAMP knock-down deregulates cytokine production and T-cell stimulatory capacity of LPS-matured dendritic cells

Figure 1

Efficiency of DC-STAMP silencing. (A) HEK293 cells were co-transfected with mDC-STAMP-GFP plasmid and one of four plasmids expressing different shRNA sequences, designed to silence DC-STAMP, or with plasmid expressing scrambled shRNA. Cells were lysed 48 hours post-transfection and lysates were subjected to Western blot analysis. Blots were stained for GFP and re-probed for β-actin as an internal reference. Data shown are representative of two independent experiments. (B) Murine BMDCs were transduced with lentivirus expressing shST1 or shST4 to silence DC-STAMP, or shScr as a negative control. Messenger RNA expression of DC-STAMP was assessed by qPCR. Levels of normalized DC-STAMP mRNA expression in cells infected with shST1 and shST4 lentiviruses were related to normalized DC-STAMP mRNA expression in shScr lentivirus infected cells. Data are depicted as mean percentage of control (shScr) ± SEM of three independent experiments.

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BMC Immunology

ISSN: 1471-2172

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