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Figure 2 | BMC Immunology

Figure 2

From: DC-STAMP knock-down deregulates cytokine production and T-cell stimulatory capacity of LPS-matured dendritic cells

Figure 2

DC-STAMP silencing does not induce phenotypic changes in mBMDCs. Murine BMDCs were infected with shScr and shST1 lentivirus at day 6 and day 7 of culture. (A) Light field microscopy image of mBMDCs in culture 4 days post-infection. (B) Lentiviraly transduced mBMDCs stained with antibody against calreticulin (green) to visualize ER and DAPI staining of nucleus (blue) analyzed by CLSM. (C) Flow cytometric analysis of CD80, CD86 and MHC class II surface expression in CD11c+ immature (-LPS) and LPS-matured (+LPS) non-infected (no virus), shScr and shST1 mBMDCs. Isotype controls are indicated by shaded area. Data shown are representative of two or three independent experiments.

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